IGEM:Peking/2007/Count-Conjugation-Notebook/2007-8-11

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Revision as of 08:34, 11 August 2007 by Anting Xu (talk | contribs) (New page: =oriT Knock Out= *By Xu Anting ==Transformation of pKO3-oriT-deleted plasmid into DH5a== *Result: negative controls have an approximately number of clones in comparing with ligation produc...)
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oriT Knock Out

  • By Xu Anting

Transformation of pKO3-oriT-deleted plasmid into DH5a

  • Result: negative controls have an approximately number of clones in comparing with ligation products. It seems that our DH5a have been contaminated.

Ligation Products Verification

  • By Colony PCR
  • Picked colonies and grew them in an additional Cm Petri-dish, in 37 centrigrade, overnight.
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