IGEM:Peking/2007/Count-Conjugation-Notebook/2007-8-11
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Tandem-OriT by Qu Mingzhi
OUR FIRST Biobricks !!
- F-OriT-J23066-OriT pSB1A2 now named as I741051!
- waiting for sequencing to get the final detail.
re-do: PlacI -> I741051
- basic experiment condition :IGEM:Peking/2007/Count-Conjugation-Notebook/2007-8-10
- we changed ligation time : from 20min ->10 min @16℃
- digesting for longer time.
electrophoresis result
oriT Knock Out
- By Xu Anting
Transformation of pKO3-oriT-deleted plasmid into DH5a
- Result: negative controls have an approximately number of clones in comparing with ligation products. It seems that our DH5a have been contaminated.
Ligation Products Verification
- By Colony PCR
- Picked colonies and grew them in an additional Cm Petri-dish, in 37 centrigrade, overnight.
