IGEM:Peking/2007/Count-Conjugation-Notebook/2007-8-13

Tandem Ori-T by Qu Mingzhi

Trimethoprim(TLC) antibiotic Efficiency Test

• new antibiotic,test efficiency, use Dh5α+pSB1A2(Amp+) as control.
• Preservative：1mg/mL in 2.5% acetic acid.

TLC(working solution):   0.5ug/mL  1ug/mL  2ug/mL  3ug/mL  4ug/mL  5ug/mL     LB-  amp+ 
R751(TLC+):                ++§       ++      ++      ++       ++     ++        +    /
Dh5α+pSB1A2(Amp+):         +¢        <+      <<+    <<<+     <<<<+  <<<<<+     +    +
empty:                     -         /        /       /        /     -         -    /

• §:grown
• ¢:can be seen by centrifugal.
• <+ means less than + by centrifugal.