IGEM:Peking/2007/Count-Conjugation-Notebook/2007-8-15: Difference between revisions
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Qu Mingzhi (talk | contribs) (New page: =Tandem OriT by Qu Mingzhi= ==transformation R-OriT & S-OriT== *Use pEASY-3 as cloning vector .(Amp+) *tramsformation system contains :3uL PCR product(after purflication), 1uL pEasy-T *...) |
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*the Culture Dish(LB/Amp+) are trated with 5uL 1M IPTG & 40uL 40mg/mL x-Gal. | *the Culture Dish(LB/Amp+) are trated with 5uL 1M IPTG & 40uL 40mg/mL x-Gal. | ||
*NEXT DAY: 10+ white cloney received. | *NEXT DAY: 10+ white cloney received. | ||
==Amplification Culture of E0240== | |||
*select Positive E0240 Colonies from Plate,Culture in liquid LB,waiting for mini-prep. |
Revision as of 00:45, 17 August 2007
Tandem OriT by Qu Mingzhi
transformation R-OriT & S-OriT
- Use pEASY-3 as cloning vector .(Amp+)
- tramsformation system contains :3uL PCR product(after purflication), 1uL pEasy-T
- the Culture Dish(LB/Amp+) are trated with 5uL 1M IPTG & 40uL 40mg/mL x-Gal.
- NEXT DAY: 10+ white cloney received.
Amplification Culture of E0240
- select Positive E0240 Colonies from Plate,Culture in liquid LB,waiting for mini-prep.