IGEM:Peking/2007/Count-Conjugation-Notebook/2007-8-15

Tandem OriT by Qu Mingzhi

transformation R-OriT & S-OriT

• Use pEASY-3 as cloning vector .(Amp+)
• tramsformation system contains :3uL PCR product(after purflication), 1uL pEasy-T
• the Culture Dish(LB/Amp+) are trated with 5uL 1M IPTG & 40uL 40mg/mL x-Gal.
• NEXT DAY: 10+ white cloney received.

Amplification Culture of E0240

• select Positive E0240 Colonies from Plate,Culture in liquid LB,waiting for mini-prep.