IGEM:Peking/2007/Count-Conjugation-Notebook/2007-8-17: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
Qu Mingzhi (talk | contribs) |
Qu Mingzhi (talk | contribs) |
||
| Line 41: | Line 41: | ||
===electrophoresis result before gel extraction=== | ===electrophoresis result before gel extraction=== | ||
*from ''left'' to ''right'': | *from ''left'' to ''right'': | ||
[[Image: | [[Image:Peking_2007-8-17_S-OriT_R-OriT_E0040-pSB1A2.jpg]] | ||
===electrophoresis result after gel extraction=== | ===electrophoresis result after gel extraction=== | ||
*from ''left'' to ''right'': | *from ''left'' to ''right'': | ||
[[Image:sample.jpg]] | [[Image:sample.jpg]] | ||
Revision as of 21:22, 17 August 2007
Tandem OriT by Qu Mingzhi
mini-prep R-OriT, S-Orit
- using Transgen mini plasmid puriflication kit.
- 50µL after purflication
Double digesting test for R-OriT, S-OriT, E0040
- use Double digesting test for gel extraction.
- R-OriT digestion system contains(Standard Assembly fragment):
4 µl 10*H buffer 1 µl EcoRI 1 µl PstI 20 µl Plasmid 14 µl dH20 -------------------------- 40 µl Total
- S-OriT digestion system contains(Standard Assembly fragment):
4 µl 10*H buffer 1 µl EcoRI 1 µl PstI 20 µl Plasmid 14 µl dH20 -------------------------- 40 µl Total
- E0040 digestion system contains(Standard Assembly vector):
4 µl 10*H buffer 1 µl EcoRI 1 µl PstI 20 µl Plasmid 14 µl dH20 -------------------------- 40 µl Total
electrophoresis result before gel extraction
- from left to right:
electrophoresis result after gel extraction
- from left to right:
