IGEM:Peking/2007/Count-Conjugation-Notebook/2007-8-17
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Tandem OriT by Qu Mingzhi
mini-prep R-OriT, S-Orit
- using Transgen mini plasmid puriflication kit.
- 50µL after purflication
Double digesting test for R-OriT, S-OriT, E0040
- use Double digesting test for gel extraction.
- R-OriT digestion system contains(Standard Assembly fragment):
4 µl 10*H buffer 1 µl EcoRI 1 µl PstI 20 µl Plasmid 14 µl dH20 -------------------------- 40 µl Total
- S-OriT digestion system contains(Standard Assembly fragment):
4 µl 10*H buffer 1 µl EcoRI 1 µl PstI 20 µl Plasmid 14 µl dH20 -------------------------- 40 µl Total
- E0040 digestion system contains(Standard Assembly vector):
4 µl 10*H buffer 1 µl EcoRI 1 µl PstI 20 µl Plasmid 14 µl dH20 -------------------------- 40 µl Total
electrophoresis result before gel extraction
- from left to right:
electrophoresis result after gel extraction
- from left to right: