IGEM:Peking/2007/Count-Conjugation-Notebook/2007-8-18: Difference between revisions

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40  µl      Total
40  µl      Total
</pre>
</pre>
====electrophoresis result====
* from ''left'' to ''right'':

Revision as of 22:39, 18 August 2007

Tandem Ori-T by Qu Mingzhi, Ren Ze

Amplification Culture of R-OriT-pSB1A2 & S-OriT-pSB1A2

  • select Positive R-OriT & S-OriT Colonies from Plate,Culture in liquid LB, waiting for mini-prep.

mini-prep PlacI-I741051-E0240_pSB1A2, R0010-E0240_pSB1A2

  • using Transgen mini plasmid puriflication kit.
  • 50µL after purflication.

mini-prep double digesting test

  • Digesting E0240 with EcoRI/PstI.
  • E0240 Digestion system contains：
1    µl      10*H
0.25 µl      EcoRI
0.25 µl      PstI
5    µl      Plasmid
3.5  µl      dH20
--------------------------
40   µl      Total

electrophoresis result

  • from left to right:
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