IGEM:Peking/2007/Count-Conjugation-Notebook/2007-8-18
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Tandem Ori-T by Qu Mingzhi, Ren Ze
Amplification Culture of R-OriT-pSB1A2 & S-OriT-pSB1A2
- select Positive R-OriT & S-OriT Colonies from Plate,Culture in liquid LB, waiting for mini-prep.
mini-prep PlacI-I741051-E0240_pSB1A2, R0010-E0240_pSB1A2
- using Transgen mini plasmid puriflication kit.
- 50µL after purflication.
mini-prep double digesting test
- Digesting E0240 with EcoRI/PstI.
- E0240 Digestion system contains：
1 µl 10*H 0.25 µl EcoRI 0.25 µl PstI 5 µl Plasmid 3.5 µl dH20 -------------------------- 40 µl Total
electrophoresis result
- from left to right:
