IGEM:Peking/2007/Count-Conjugation-Notebook/2007-8-19: Difference between revisions
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===electrophoresis result === | ===electrophoresis result === | ||
==colony PCR== | ==colony PCR Test for R751, pSC101== | ||
*Test Lb- R751 plate, Lb- pSC101 have the correct plasmid. | |||
*Test plate:LB- R751, Lb- pSB101, Tc+ R751-pSC101, Tc+ Dh5+pSC101 | |||
Revision as of 22:44, 19 August 2007
Tandem Ori-T by Qu Mingzhi, Ren Ze
mini-prep R-OriT, S-Orit (pSB1A2)
- using Transgen mini plasmid puriflication kit.
- 50µL after purflication
mini-prep double digesting test
- R-OriT, S-OriT digestion system contains(Standard Assembly fragment):
4 µl 10*H buffer 1 µl EcoRI 1 µl PstI 20 µl Plasmid 14 µl dH20 -------------------------- 40 µl Total
electrophoresis result
colony PCR Test for R751, pSC101
- Test Lb- R751 plate, Lb- pSC101 have the correct plasmid.
- Test plate:LB- R751, Lb- pSB101, Tc+ R751-pSC101, Tc+ Dh5+pSC101
