IGEM:Peking/2007/Count-Conjugation-Notebook/2007-8-19

Tandem Ori-T by Qu Mingzhi, Ren Ze

mini-prep R-OriT, S-Orit (pSB1A2)

• using Transgen mini plasmid puriflication kit.
• 50µL after purflication

mini-prep double digesting test

• R-OriT, S-OriT digestion system contains(Standard Assembly fragment):

4 µl       10*H buffer
1 µl       EcoRI
1 µl       PstI
20 µl      Plasmid
14 µl      dH20
--------------------------
40 µl      Total