IGEM:Peking/2007/Count-Conjugation-Notebook/2007-8-2: Difference between revisions
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*OriT by Qu Mingzhi | *OriT by Qu Mingzhi | ||
==mini-prep F- | ==mini-prep F-OriT_pEasy-T3== | ||
*using Transgen mini plasmid puriflication kit. | |||
===mini-prep result=== | |||
*from ''left'' to ''right'': | |||
#mini-prep F-OriT_pEasy-T3-1 | |||
#mini-prep F-OriT_pEasy-T3-2 | |||
#marker | |||
===mini-prep double digesting test result=== | |||
*Digesting F-OriT_pEasy-T3 with EcoR1/PstI. | |||
*Digestion system contains (totally 10uL): 1uL 10*H, 0.25uL EcoRI, 0.25uL PstI, 5uL plasmid, 3.5uL ddH2O. | |||
37℃ culutre for 3 hours. |
Revision as of 20:19, 2 August 2007
- OriT by Qu Mingzhi
mini-prep F-OriT_pEasy-T3
- using Transgen mini plasmid puriflication kit.
mini-prep result
- from left to right:
- mini-prep F-OriT_pEasy-T3-1
- mini-prep F-OriT_pEasy-T3-2
- marker
mini-prep double digesting test result
- Digesting F-OriT_pEasy-T3 with EcoR1/PstI.
- Digestion system contains (totally 10uL): 1uL 10*H, 0.25uL EcoRI, 0.25uL PstI, 5uL plasmid, 3.5uL ddH2O.
37℃ culutre for 3 hours.