IGEM:Peking/2007/Count-Conjugation-Notebook/2007-8-2

• OriT by Qu Mingzhi

mini-prep F-OriT_pEasy-T3

• using Transgen mini plasmid puriflication kit.

mini-prep result

• from left to right:

1. mini-prep F-OriT_pEasy-T3-1
2. mini-prep F-OriT_pEasy-T3-2
3. marker

mini-prep double digesting test result

• Digesting F-OriT_pEasy-T3 with EcoR1/PstI.
• Digestion system contains (totally 10uL): 1uL 10*H, 0.25uL EcoRI, 0.25uL PstI, 5uL plasmid, 3.5uL ddH2O.
• 37℃ culutre for 3 hours.
• from left to right:

1. mini-prep F-OriT_pEasy-T3-1 @ EcoRI/PstI
2. mini-prep F-OriT_pEasy-T3-2 @ EcoRI/PstI
3. marker 1
4. marker 2

• I think the channel is bright enough for purification,so.... ^_^

Ori-T purification

• use Transgen kit.
• 30uL after purflication

electrophorsis result

• from left to right:

1. purified F-Ori-T
2. marker