IGEM:Peking/2007/Count-Conjugation-Notebook/2007-8-20
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Tandem OriT by Qu Mingzhi & Ren Ze
Preparation of Competent Cells: F+, R751
- Preparation F+, R751 competent cell for Conjugation test.
mini-prep PlacI-I741051-E0240_pSB1A2, R0010-E0240_pSB1A2 (II)
- using Transgen mini plasmid puriflication kit.
- 50µL after purflication.
mini-prep double digesting test
- Digesting PlacI-I741051-E0240_pSB1A2, R0010-E0240_pSB1A2 with EcoRI/PstI.
- Digestion system contains：
1 µl 10*H 0.25 µl EcoRI 0.25 µl PstI 5 µl Plasmid 3.5 µl dH20 -------------------------- 40 µl Total
electrophoresis result
- from left to right:
