IGEM:Peking/2007/Count-Conjugation-Notebook/2007-8-21
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Tandem OriT by Qu Mingzhi
Ligation: PlacI-I741051 <- E0240 & R0010 <- E0240 (III)
- Ligate the E0240 fragment and PlacI-I741051 vector .
- Ligate the E0240 fragment and R0010 vector .
- use super fast T4 DNA ligase
- super fast T4 DNA ligase Ligation system contains:
2 µl vector 3 µl E0240 fragment 0.5 µl ''super fast T4 DNA ligase'' 1 µl 10X T4 ligase buffer 3.5 µl dH20 -------------------------- 10 µl Total
transformation PlacI-I741051 <- E0240 & R0010 <- E0240(II)
NEXT DAY: Received clones.