IGEM:Peking/2007/Count-Conjugation-Notebook/2007-8-3: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
Qu Mingzhi (talk | contribs) |
No edit summary |
||
Line 1: | Line 1: | ||
=Tandem OriT by Qu Mingzhi= | |||
==Amplification Culture of F-OriT->pSB1A2== | ==Amplification Culture of F-OriT->pSB1A2== | ||
*Culture at Amp+ plate for 12 hours. | *Culture at Amp+ plate for 12 hours. | ||
*select Positive OriT-pSB1A2 Colonies from Plate,Culture in liquid LB,waiting for mini-prep. | *select Positive OriT-pSB1A2 Colonies from Plate,Culture in liquid LB,waiting for mini-prep. | ||
*pSB1A2 self-ligation nagetive-control did not grow. | *pSB1A2 self-ligation nagetive-control did not grow. |
Revision as of 04:54, 9 August 2007
Tandem OriT by Qu Mingzhi
Amplification Culture of F-OriT->pSB1A2
- Culture at Amp+ plate for 12 hours.
- select Positive OriT-pSB1A2 Colonies from Plate,Culture in liquid LB,waiting for mini-prep.
- pSB1A2 self-ligation nagetive-control did not grow.