IGEM:Peking/2007/Count-Conjugation-Notebook/2007-8-3

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Tandem OriT by Qu Mingzhi

Amplification Culture of F-OriT->pSB1A2

  • Culture at Amp+ plate for 12 hours.
  • select Positive OriT-pSB1A2 Colonies from Plate,Culture in liquid LB,waiting for mini-prep.
  • pSB1A2 self-ligation nagetive-control did not grow.
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