IGEM:Peking/2007/Count-Conjugation-Notebook/2007-8-3

From OpenWetWare
Revision as of 07:03, 24 August 2007 by Anting Xu (talk | contribs)
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)
Jump to navigationJump to search
The printable version is no longer supported and may have rendering errors. Please update your browser bookmarks and please use the default browser print function instead.

Tandem OriT by Qu Mingzhi

Amplification Culture of F-OriT->pSB1A2

  • Culture at Amp+ plate for 12 hours.
  • select Positive OriT-pSB1A2 Colonies from Plate,Culture in liquid LB,waiting for mini-prep.
  • pSB1A2 self-ligation nagetive-control did not grow.

Lock & Key By Yu Tao

oriT Knock Out

  • By Xu Anting

Sequencing results

  • Got sequencing-corrected strains of F and R751.
  • All pSC101 sent for sequencing (7 colonies) have the same point mutations in several regions. I wonder if our strain from CGMCC is different from that in NCBI database. I will go on with these strains and meanwhile repeat the oriT-pSC101-deleted construction.

Amplification culture of FRS

  • Amplification culture of sequenced strains: F (No. 3); R751 (No. 3); pSC101 (No. 4). Shake with LB-Amp+ liquid in 37℃ for 16 hours.
Retrieved from "https://openwetware.org/mediawiki/index.php?title=IGEM:Peking/2007/Count-Conjugation-Notebook/2007-8-3&oldid=144644"