IGEM:Peking/2007/Count-Conjugation-Notebook/2007-8-7

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Tandem OriT by Qu Mingzhi

Amplification Culture of OriT_J23066_OriT_pSB1A2(fast T4 ligase)

  • select Positive OriT_J23066_OriT_pSB1A2 Colonies from Plate, Culture in liquid LB,waiting for mini-prep.

transformation OriT_J23066_OriT_pSB1A2 (nomal T4 ligase)

  • transformation OriT_J23066_OriT_pSB1A2 ligate by nomal T4 ligase .
  • Culture at Amp+ plate for 12 hours.
  • NEXT DAY: 200+ cloney received.
  • F-OriT_J23066_OriT_pSB1A2 self-ligation nagetive-control received no clones.
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