IGEM:Peking/2007/Count-Conjugation-Notebook/2007-8-9: Difference between revisions
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*First, I have to say sorry because I upload the previous experiment records to our notebook so late. But late is ever better than never, right. n_n--[[User:Tao Yu|Tao Yu]] 08:00, 9 August 2007 (EDT) | *First, I have to say sorry because I upload the previous experiment records to our notebook so late. But late is ever better than never, right. n_n--[[User:Tao Yu|Tao Yu]] 08:00, 9 August 2007 (EDT) | ||
=Lock & Key by Yu Tao= | |||
==Key1 & Lock 1 Efficiency Test== | |||
*Add 1 mL overnight incubated sample culture of each group to 9 mL fresh LB. | |||
*Incubate them in 37℃ for 2 hours. | |||
*Divide the R0010.J23066(Key) and R0040.J23078.E0040.B0015-DH5a into 2 test tubes, add IPTG(finally 1uM) to only one of the two. | |||
*Culture all the samples overnight. | |||
Revision as of 23:48, 10 August 2007
- First, I have to say sorry because I upload the previous experiment records to our notebook so late. But late is ever better than never, right. n_n--Tao Yu 08:00, 9 August 2007 (EDT)
Lock & Key by Yu Tao
Key1 & Lock 1 Efficiency Test
- Add 1 mL overnight incubated sample culture of each group to 9 mL fresh LB.
- Incubate them in 37℃ for 2 hours.
- Divide the R0010.J23066(Key) and R0040.J23078.E0040.B0015-DH5a into 2 test tubes, add IPTG(finally 1uM) to only one of the two.
- Culture all the samples overnight.
