IGEM:Peking/2007/Count-Conjugation-Procedure

Make more independent riboregulators

1. We choose to use <bbpart>pSB1A3<\bbpart> and <bbpart>BBa_R0010<\bbpart>(Plac) to express the taRNA, and <bbpart>pSB3K3<\bbpart> and <bbpart>BBa_R0040<\bbpart> to express <bbpart>BBa_E0040<\bbpart>, whose translation is under the control of crRNA.
2. crRNA is synthesized as two partially overlap ~50bp long primer, and one cycle PCR can make it to a dsDNA with some BioBrick cloning sites flanked.
3. crRNA should be cut by NotI at 5' and SpeI at 3' and cloned into pSB1A3 to get the full length BioBrick prefix and suffix.
4. First, we will try out the UC Berkley riboregulators <bbpart>BBa_J23078<\bbpart> and <bbpart>BBa_J23066<\bbpart> first.
5. This is the experimental procedure.