IGEM:Peking/2007/Count:Agarose Gel Electrophoresis

Casting agarose gels

Materials

• gel box, gel tray, comb
• Agarose
• GeneFinder safe dye(200X stock)
• 1X TAE
• Microwave
• 100 ml glass bottle or beaker

Procedure

1. Assemble casting trays ,gel box and gel comb,choose the comb of appropriate width, size, and number into niche at end of the tray.
2. agarose solution(1% gel)：0.2g agarose + 20ml 1X TAE for small gels or 0.4g agarose + 40ml 1X TAE for large gels.
3. add agarose solution in a 100ml bottle or beaker.
4. Microwave bottle for 1min
5. Remove from microwave and let cool
6. Once gel is cooled so that it can be touched comfortably with your gloved hand, add 50 μL(for small gel) or 100 μL(for large gels) GeneFinder safe dye.
7. Pour gel into casting trays.

prepare Loading dye

• mix 10X loading buffer and 200X GeneFinder safe dye 1:1.
• Store at 4°C

See also

• Agarose_gel_electrophoresis
• Endy:Agarose gel
• Knight:Agarose gel electrophoresis