IGEM:Peking/2007/Count:Agarose Gel Electrophoresis
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Casting agarose gels
Materials
- gel box, gel tray, comb
- Agarose
- GeneFinder safe dye(200X stock)
- 1X TAE
- Microwave
- 100 ml glass bottle or beaker
Procedure
- Assemble casting trays ,gel box and gel comb,choose the comb of appropriate width, size, and number into niche at end of the tray.
- agarose solution(1% gel):0.2g agarose + 20ml 1X TAE for small gels or 0.4g agarose + 40ml 1X TAE for large gels.
- add agarose solution in a 100ml bottle or beaker.
- Microwave bottle for 1min
- Remove from microwave and let cool
- Once gel is cooled so that it can be touched comfortably with your gloved hand, add 50 μL(for small gel) or 100 μL(for large gels) GeneFinder safe dye.
- Pour gel into casting trays.
prepare Loading dye
- mix 10X loading buffer and 200X GeneFinder safe dye 1:1.
- Store at 4°C