IGEM:Peking/2007/Count:Gel-Extraction

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Revision as of 09:35, 9 July 2007 by Qu Mingzhi (talk | contribs) (New page: ==quick gel purifcation== according to ''Transgen'' kit protocol #Agarose gel electrophoresis,cut the DNA band as thin as possible.pute into a clear EP tube.weighing,if the weight is 100mg...)
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quick gel purifcation

according to Transgen kit protocol

  1. Agarose gel electrophoresis,cut the DNA band as thin as possible.pute into a clear EP tube.weighing,if the weight is 100mg,the volume can be

consider as 100uL,add solution GSB,put into 55C water bath until complently merge,it takes about 5 minutes

  1. cool the tube with water to adjust the tempertature of the solution to room temperature
  2. Add the solution into the collection tube, standing for 1 minute,13000rpm for 1 min, discard the flow-through
  3. Add 650 uL WB,13000rpm for 1 min,discard the flow-through
  4. 13000rpm for 2 min,discard the flow-through
  5. Put the collection tube into a clean EP tube, Add 50 uL 60C ddH2O in the center of the collection tube,standing for 1 min
  6. 13000rpm for 1 min.
  7. store the DNA solution at -20C
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