IGEM:Peking/2007/Count:PCR product purifcation: Difference between revisions
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Qu Mingzhi (talk | contribs) (New page: ==11== #Take 50-100uL PCR product solution,add 5 times volume solutoin BB,mix, add into spin column,standing for 1 min, 13000rpm for 1 min,discard the flow-through. #Add 650 mL buffer WB ,...) |
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== | ==PCR product purifcation== | ||
#Take 50-100uL PCR product solution,add 5 times volume solutoin BB,mix, add into spin column,standing for 1 min, 13000rpm for 1 min,discard the flow-through. | #Take 50-100uL PCR product solution,add 5 times volume solutoin BB,mix, add into spin column,standing for 1 min, 13000rpm for 1 min,discard the flow-through. | ||
#Add 650 mL buffer WB ,13000 rpm for 1min,discard the supernatant | #Add 650 mL buffer WB ,13000 rpm for 1min,discard the supernatant | ||
Revision as of 09:42, 9 July 2007
PCR product purifcation
- Take 50-100uL PCR product solution,add 5 times volume solutoin BB,mix, add into spin column,standing for 1 min, 13000rpm for 1 min,discard the flow-through.
- Add 650 mL buffer WB ,13000 rpm for 1min,discard the supernatant
- 13000rpm for 2 min to wipe out the remaining WB
- Put the collection tube into a clean EP tube, Add 50 uL 60C ddH2O in the center of the collection tube,standing for 1 min
- 13000rpm for 1 min.
- store the DNA solution at -20C
