Qu Mingzhi: IGEM:Peking/2007/Transformation moved to IGEM:Peking/2007/Count:Transformation: add group name:"Count"

Qu Mingzhi — Tue, 21 Aug 2007 05:40:49 GMT

IGEM:Peking/2007/Transformation moved to IGEM:Peking/2007/Count:Transformation: add group name:"Count"

←Older revision

Revision as of 05:40, 21 August 2007

Anting Xu: New page: #Hold competent cells (from -80℃ refrigerator) on ice. #Gently mix ligation product (10-15 μL) with cells, then bath in ice for 30 min. #(Precisely!) Bath in 42℃ water for 90 sec. #Ho...

Anting Xu — Tue, 21 Aug 2007 03:11:43 GMT

New page: #Hold competent cells (from -80℃ refrigerator) on ice. #Gently mix ligation product (10-15 μL) with cells, then bath in ice for 30 min. #(Precisely!) Bath in 42℃ water for 90 sec. #Ho...

New page

#Hold competent cells (from -80℃ refrigerator) on ice.
#Gently mix ligation product (10-15 μL) with cells, then bath in ice for 30 min.
#(Precisely!) Bath in 42℃ water for 90 sec.
#Hold again in ice for 2 min.
#Bath in 37℃ for 45 min. For plasmid pKO3 (and other temperature-sensitive strains), bath in 30℃ for 1 hour.
#Separate cells on petri-dishes, and cultivate them in 37℃ for 12 hours (for pKO3, 24 hours are necessary).

IGEM:Peking/2007/Count:Transformation - Revision history

Qu Mingzhi: IGEM:Peking/2007/Transformation moved to IGEM:Peking/2007/Count:Transformation: add group name:"Count"

Anting Xu: New page: #Hold competent cells (from -80℃ refrigerator) on ice. #Gently mix ligation product (10-15 μL) with cells, then bath in ice for 30 min. #(Precisely!) Bath in 42℃ water for 90 sec. #Ho...