IGEM:Peking/2007/Switch-Notebook/2007-7-14

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Revision as of 02:49, 14 July 2007 by Chen Daizhuo (talk | contribs) (New page: Experimenter: CDZ, MHS, LC, CXY ==Contents== ===Precipitate with ethanol: pCC002^^ (NotI/XhoI)=== * Time: 8:15am ===Ligation of SDA-H, Y, cro, lacZa with pCC002^^=== * Time: 2pm * SDA-H,...)
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Experimenter: CDZ, MHS, LC, CXY

Contents

Precipitate with ethanol: pCC002^^ (NotI/XhoI)

  • Time: 8:15am

Ligation of SDA-H, Y, cro, lacZa with pCC002^^

  • Time: 2pm
  • SDA-H, Y, cro, lacZa: 4uL
  • pCC002^^: 2uL

Miniprep and digestion test of pLX007-lacZa(1-53)

  • H buffer: 2uL
  • XhoI: 0.5uL
  • NdeI: 0.5uL
  • Plasmid: 10uL
  • ddH2O: 7uL
  • total: 20uL

l to r: pLX007-lacZa(1-23,25), marker

胶倒的不好。没连上应该看到300bp的带，连上了应该有500bp。都没有！质粒太少了…

Sequencing result

CD1-2,4,5, SS1-2 are correct. Great! We have CD template.

RD1 is wrong. New primers already sent.

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