IGEM:Peking/2007/Switch-Notebook/2007-7-14

From OpenWetWare

< IGEM:Peking | 2007
Revision as of 08:37, 14 July 2007 by Chen Daizhuo (Talk | contribs)
(diff) ←Older revision | Current revision (diff) | Newer revision→ (diff)
Jump to: navigation, search

Experimenter: CDZ, MHS, LC, CXY

Contents

Contents

Transformation of SDA-H, Y, cro, lacZa-pCC002

  • 8:15am, precipitate with ethanol: pCC002^^ (NotI/XhoI)
  • 2:00pm, ligation of SDA-H, Y, cro, lacZa-pCC002 with pCC002^^ (4uL/1uL)
  • 8:30pm, transformation

Sequencing result

CD1-2,4,5, SS1-2 are correct. Great! We have CD template.

RD1 is wrong. New primers already sent.

Miniprep and digestion test of pLX007-lacZa(1-53)

  • H buffer: 2uL
  • XhoI: 0.5uL
  • NdeI: 0.5uL
  • Plasmid: 10uL
  • ddH2O: 7uL
  • total: 20uL

Image:PKU Switch 07-7-14 PLX007-lacZa(1-23,25), marker.gif

l to r: pLX007-lacZa(1-23,25), marker

胶倒的不好。没连上应该看到300bp的带,连上了应该有500bp。都没有!质粒太少了?质粒不对?

Digestion test of pLX007(1)

Image:PKU Switch 07-7-14 PLX007^^(NdeI,ScaI).gif

l to r: pLX007(1)^^(NdeI/ScaI), marker

Not digested. The plasmid is wrong???

Personal tools