IGEM:Peking/2007/Switch-Notebook/2007-7-16
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Experimenter: NM & LCB
Contents
pLX007挑菌
Colony PCR of CD2up/down-pMD18T
- Primers: MBF/R
Select for incubation & sequencing: 2,4,7,8.
Miniprep of SD, lacZa, pCC002, A-H, Y, cro
Double digestion test of BamHI, XhoI (plasmid: 10uL, digestion time: 2.5hrs)
Temperature gradient PCR of CD3
- template: CD1-pMD18T 1uL
- buffer: 5uL
- dNTP: 5uL
- F: Con_D_3_F 1uL
- R: Rec_S_3_R 1uL
- Extaq: 0.25uL
- H2O: 37uL
- temperature: 50.3, 53.2, 58.1, 63.5, 68.1
Single digestion test of pLX008(2uL), pLX009(4uL)
- digestion test: 2.5hr