IGEM:Peking/2007/Switch-Notebook/2007-7-19: Difference between revisions
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Chen Daizhuo (talk | contribs) (New page: Experimenter: CXY, CCY ==Contents== ===Colony PCR test of pCC002, Mach1-T1(1-9), JM110(1-9)=== ===Colony PCR test of SS1-pMD18T-3, CD1-pMD18T-3=== Plasmids are diluted to 1/50 of concent...) |
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[[Image:PKU Switch 07-07-19 Plx-lacZ alpha pcr 1-23.jpg]] | [[Image:PKU Switch 07-07-19 Plx-lacZ alpha pcr 1-23.jpg]] | ||
pLX007-lacZa(1-21), pLX007 - Control, Marker, pLX008-lacZa(22-23) | pLX007-lacZa(1-21), pLX007 - Control, Marker, pLX008-lacZa(22-23) | ||
[[Image:PKU Switch 07-07-19 Plx-lacZ alpha pcr 24-47.jpg]] | [[Image:PKU Switch 07-07-19 Plx-lacZ alpha pcr 24-47.jpg]] | ||
pLX008-lacZa(24-41), Marker, pLX008-lacZa(42-47) | pLX008-lacZa(24-41), Marker, pLX008-lacZa(42-47) | ||
[[Image:PKU Switch 07-07-19 Plx-lacZ alpha pcr 48-70.jpg]] | [[Image:PKU Switch 07-07-19 Plx-lacZ alpha pcr 48-70.jpg]] | ||
pLX008-lacZa(48-66), pLX008 – Control, Marker, pLX009-lacZa(67-70) | pLX008-lacZa(48-66), pLX008 – Control, Marker, pLX009-lacZa(67-70) | ||
[[Image:PKU Switch 07-07-19 Plx-lacZ alpha pcr 71-93.jpg]] | [[Image:PKU Switch 07-07-19 Plx-lacZ alpha pcr 71-93.jpg]] | ||
pLX009-lacZa(71-93), pLX009 – Control, Marker | pLX009-lacZa(71-93), pLX009 – Control, Marker | ||
Latest revision as of 08:15, 19 July 2007
Experimenter: CXY, CCY
Contents
Colony PCR test of pCC002, Mach1-T1(1-9), JM110(1-9)
Colony PCR test of SS1-pMD18T-3, CD1-pMD18T-3
Plasmids are diluted to 1/50 of concentration before PCR.
- Primers: Sul_S_1_F, Sul_S_1_R
- Enzyme: Easy Taq
OK!! Incubate for Miniprep and digestion test tomorrow.
Colony PCR test of pLX007/8/9-lacZa
- Primers: test_F_new, test_R
- Enzyme: Easy Taq
pLX007-lacZa(1-21), pLX007 - Control, Marker, pLX008-lacZa(22-23)
pLX008-lacZa(24-41), Marker, pLX008-lacZa(42-47)
pLX008-lacZa(48-66), pLX008 – Control, Marker, pLX009-lacZa(67-70)
pLX009-lacZa(71-93), pLX009 – Control, Marker
pLX007-lacZa(8), pLX008-lacZa(40,41), pLX009-lacZa(68,88) OK!! Incubate for Miniprep and digestion test tomorrow.
