IGEM:Peking/2007/Switch-Notebook/2007-7-21: Difference between revisions
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Chen Chongyi (talk | contribs) (New page: ==PCR RS1,2,3== ddH2O 36.75uL Buffer 5uL dNTP 5uL pRec7-8 1uL Rec-S-1-F 1uL Rec-S-1-Rnew 1uL Ex-Taq 0.25uL total 50uL temperature grad: 53.2, 58.5, 63.5, 68.1 result: RS1 and RS2'...) |
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Latest revision as of 07:10, 26 October 2007
PCR RS1,2,3
ddH2O 36.75uL
Buffer 5uL
dNTP 5uL
pRec7-8 1uL
Rec-S-1-F 1uL
Rec-S-1-Rnew 1uL
Ex-Taq 0.25uL
total 50uL
temperature grad: 53.2, 58.5, 63.5, 68.1
result: RS1 and RS2's production got but RS3 failed.
transformation of pRec 7-8
Mistake about antibiotic, failed
miniprep of plx007/008/009-lacZa
double digestion test
H buffer 2uL
XhoI/SalI 0.5uL
plasmid 7uL
dH2O 10uL
total 20uL
eletrophoresis result: no visible band
preparation of LB plates with Amp
double digestion of plx007, plx008, plx009
H buffer 2uL
ScaI/SalI 0.5uL
plasmid 7uL
dH2O 10uL
total 20uL
electrophoresis result: digestion not successful, but the plasmid is right.
