IGEM:Peking/2007/Switch-Notebook/2007-7-23

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Experimenter: CXY

We have been trying to connect lacZa into pLX007/8/9 for three weeks but still failed. We decide to repeat the entire process carefully.

Contents

Contents

Overnight ligation of pLX008-lacZa

pLX008 and lacZa are both digested by XhoI and SalI.

Double digestion of pLX007/8/9 with XhoI and SalI

Double digestion of lacZa-pMD18T with XhoI and SalI

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