IGEM:Peking/2007/Switch-Notebook/2007-7-24
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Experimenter: LD & CXY
Contents
Precipitate to retract: pLX007/8/9^^(XhoI/SalI), lacZa^^(XhoI/SalI)
Overnight ligation of pLX007/8/9^^ and lacZa^^
- Plasmid: 4.5uL, lacZa: 0.5uL
Retract from electrophoresis jel: lacZa-T^(XhoI/SalI)
Not retracted because the concentration is too low.
Temperature PCR of RS1-3, RD1-3 from Prec-T
- Template: 1uL
- Buffer: 5uL
- dNTP: 5uL
- Primer_F Rec_S_1_F or Rec_D_1_F: 1uL
- Primer_R Rec_S_1_Rnew: 1uL
- Ex-taq: 0.25uL
- H2O: 37uL
- Total: 50.25uL
- Product: RS1, RD1
- Temperature gradient: 53.2C, 58.1C, 63.5C, 68.1C
l to r: RD1(1-4), RS1(1-4), 100kb ladder
retract: RS1(53.2C), RD1(53.2C)
- Template: 1uL
- Buffer: 5uL
- dNTP: 5uL
- Primer_F Rec_S_2/3_F or Rec_D_2/3_F: 1uL
- Primer_R Rec_S_2/3_Rnew: 1uL
- Ex-taq: 0.25uL
- H2O: 37uL
- Total: 50.25uL
- Product: RS2/3, RD2/3
- Temperature gradient: 68C
l to r: RS2, RS3, RD2, RD3, 100kb ladder
retract: RS2, RD2
