IGEM:Peking/2007/Switch-Notebook/2007-7-24

From OpenWetWare
Revision as of 07:34, 27 July 2007 by Chen Daizhuo (talk | contribs) (New page: Experimenter: LD & CXY ==Contents== ===Precipitate to retract: pLX007/8/9^^(XhoI/SalI), lacZa^^(XhoI/SalI)=== ===Overnight ligation of pLX007/8/9^^ and lacZa^^=== * Plasmid: 4.5uL, lacZa...)
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)
Jump to navigationJump to search
The printable version is no longer supported and may have rendering errors. Please update your browser bookmarks and please use the default browser print function instead.

Experimenter: LD & CXY

Contents

Precipitate to retract: pLX007/8/9^^(XhoI/SalI), lacZa^^(XhoI/SalI)

Overnight ligation of pLX007/8/9^^ and lacZa^^

  • Plasmid: 4.5uL, lacZa: 0.5uL

Retract from electrophoresis jel: lacZa-T^(XhoI/SalI)

Not retracted because the concentration is too low.

Temperature PCR of RS1-3, RD1-3 from Prec-T

  • Template: 1uL
  • Buffer: 5uL
  • dNTP: 5uL
  • Primer_F Rec_S_1_F or Rec_D_1_F: 1uL
  • Primer_R Rec_S_1_Rnew: 1uL
  • Ex-taq: 0.25uL
  • H2O: 37uL
  • Total: 50.25uL
  • Product: RS1, RD1
  • Temperature gradient: 53.2C, 58.1C, 63.5C, 68.1C

l to r: RD1(1-4), RS1(1-4), 100kb ladder

retract: RS1(53.2C), RD1(53.2C)

  • Template: 1uL
  • Buffer: 5uL
  • dNTP: 5uL
  • Primer_F Rec_S_2/3_F or Rec_D_2/3_F: 1uL
  • Primer_R Rec_S_2/3_Rnew: 1uL
  • Ex-taq: 0.25uL
  • H2O: 37uL
  • Total: 50.25uL
  • Product: RS2/3, RD2/3
  • Temperature gradient: 68C

l to r: RS2, RS3, RD2, RD3, 100kb ladder

retract: RS2, RD2

Overnight ligation of RS1, RD1, RS2, RD2 with pMD18-T

Retrieved from "https://openwetware.org/mediawiki/index.php?title=IGEM:Peking/2007/Switch-Notebook/2007-7-24&oldid=134690"