IGEM:Peking/2007/Switch-Notebook/2007-7-26: Difference between revisions
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Experimenter: LC, CCY | |||
==Contents== | |||
===Colony PCR test of lacZa-pLX007/8/9=== | |||
* Primers: test_F_new, test_R | |||
30 colonies are tested for each of lacZa-pLX007/8/9, 2 self connections are used as control. 96 in total. | |||
延伸45s, 退火60C, 25 cycles. | |||
Only lacZa-pLX009(20) is right. lacZa-pLX008 is probably right, but the size seems wrong. Both are incubated at 2pm. | |||
===PCR of CD2/3 from CD1-pMD18-T=== | |||
CD2 is retracted. CD3 failed. | |||
===PCR of lacZa=== | |||
Retracted. | |||
===Ligation and transformation of CD2-pMD18-T=== | |||
===Single digestion of pLX007/8/9 with SalI or XhoI=== | |||
SalI is OK. XhoI can not digest the plasmid. | |||
Latest revision as of 08:13, 27 July 2007
Experimenter: LC, CCY
Contents
Colony PCR test of lacZa-pLX007/8/9
- Primers: test_F_new, test_R
30 colonies are tested for each of lacZa-pLX007/8/9, 2 self connections are used as control. 96 in total.
延伸45s, 退火60C, 25 cycles.
Only lacZa-pLX009(20) is right. lacZa-pLX008 is probably right, but the size seems wrong. Both are incubated at 2pm.
PCR of CD2/3 from CD1-pMD18-T
CD2 is retracted. CD3 failed.
PCR of lacZa
Retracted.
Ligation and transformation of CD2-pMD18-T
Single digestion of pLX007/8/9 with SalI or XhoI
SalI is OK. XhoI can not digest the plasmid.
