IGEM:Peking/2007/Switch-Notebook/2007-7-27: Difference between revisions
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Chen Chongyi (talk | contribs) (New page: ==Re-digestion of plx007, plx008 and plx009== Plasmid 2uL XhoI 0.5uL H buffer 2uL ddH2O 15.5uL electrophoresis result: invisible ==pick colony of plx008, plx009== ==rescue by precipitat...) |
Chen Chongyi (talk | contribs) |
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==Re-digestion of plx007, plx008 and plx009== | ==Re-digestion of plx007, plx008 and plx009== | ||
Plasmid 2uL | Plasmid 2uL | ||
XhoI 0.5uL | XhoI 0.5uL | ||
H buffer 2uL | H buffer 2uL | ||
ddH2O 15.5uL | ddH2O 15.5uL | ||
Latest revision as of 07:28, 26 October 2007
Re-digestion of plx007, plx008 and plx009
Plasmid 2uL
XhoI 0.5uL
H buffer 2uL
ddH2O 15.5uL
electrophoresis result: invisible
pick colony of plx008, plx009
rescue by precipitation of LacZa
pick lacZa-T positive transformation colony and miniprep
Colony PCR of CD2-T
Preparation of LB medium with Cm
Re-PCR of LacZa and CD2, CD3
primer-F/R 1uL each
Ex-Buffer 5uL
dNTP 5uL
Ex-Taq 0.25uL
ddH2O 36.75uL
60 degrees and 35 cycles with temperature grad
==single digestion of plx007, 008, 009 Plasmid 4uL
XhoI 0.5uL
H buffer 2uL
ddH2O 15.5uL
miniprep of lacZa-T using positive transformation colony and digestion
plasmid 10uL
XhoI/SalI 0.5uL
H buffer 2uL
ddH2O 7uL
total 20uL
digestion for 1 hour and electrophoresis test for the result.