IGEM:Peking/2007/Switch-Notebook/2007-7-27
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Re-digestion of plx007, plx008 and plx009
Plasmid 2uL
XhoI 0.5uL
H buffer 2uL
ddH2O 15.5uL
electrophoresis result: invisible
pick colony of plx008, plx009
rescue by precipitation of LacZa
pick lacZa-T positive transformation colony and miniprep
Colony PCR of CD2-T
Preparation of LB medium with Cm
Re-PCR of LacZa and CD2, CD3
primer-F/R 1uL each
Ex-Buffer 5uL
dNTP 5uL
Ex-Taq 0.25uL
ddH2O 36.75uL
60 degrees and 35 cycles with temperature grad
==single digestion of plx007, 008, 009 Plasmid 4uL
XhoI 0.5uL
H buffer 2uL
ddH2O 15.5uL
miniprep of lacZa-T using positive transformation colony and digestion
plasmid 10uL
XhoI/SalI 0.5uL
H buffer 2uL
ddH2O 7uL
total 20uL
digestion for 1 hour and electrophoresis test for the result.