IGEM:Peking/2007/Switch-Notebook/2007-7-28

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Contents

rescue of digestion production of XhoI/SalI

double digestion test of plx008/009-lacZa

using two systems of ScaI/SalI and XhoI/ScaI

eletrophoresis to check the result

Pick 12 colonies of pcc009-MCS and cultivate

lacZa-T single digestion test by SalI or XhoI

RS1-3, 1-6, 1-8, 2-10 and RD1-1 miniprep by kit and stored

single digestion of RD and RS

plasmid 3uL

SalI 0.5uL

H buffer 2uL

ddH2O 14.5uL

Colony PCR of CD2-T

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