IGEM:Peking/2007/Switch-Notebook/2007-7-6: Difference between revisions
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===SDA-EGFP-pCC002(No.1,5)阳转长起来了=== | ===SDA-EGFP-pCC002(No.1,5)阳转长起来了=== | ||
但提质粒用的是存下来的菌液, BamHI/XhoI(7uL/20uL)切检验, OK! 存质粒. | 但提质粒用的是存下来的菌液, BamHI/XhoI(7uL/20uL)切检验, OK! 存质粒. | ||
[[Image:PKU Switch 07-7-6 SDA-EGFP-pCC002(1,5).gif]] | |||
还有菌液在冰箱门上存着, 用时直接提. | 还有菌液在冰箱门上存着, 用时直接提. | ||
Revision as of 07:12, 6 July 2007
Experimenter: YT, YL, LCB, WMC
Contents
乙醇沉淀pLX007(SalI/XhoI), pCC009(SalI/XhoI) and lacZa(SalI/XhoI)
2倍体积乙醇, 1/10体积NaAc.
回收于20uL, 离心30min.
SDA-EGFP-pCC002(No.1,5)阳转长起来了
但提质粒用的是存下来的菌液, BamHI/XhoI(7uL/20uL)切检验, OK! 存质粒.
.gif)
还有菌液在冰箱门上存着, 用时直接提.
