IGEM:Peking/2007/Switch-Notebook/2007-7-6: Difference between revisions
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* MCS-pCC009(4uL/1uL), H20-pCC009自连 | * MCS-pCC009(4uL/1uL), H20-pCC009自连 | ||
[[Image:PKU_Switch_07-7-6_lazZa xs.gif]] | |||
5pm-6pm: 4 degree, 6pm-9pm 16 degree. | 5pm-6pm: 4 degree, 6pm-9pm 16 degree. | ||
Revision as of 07:44, 6 July 2007
Experimenter: YT, YL, LCB, WMC
Contents
乙醇沉淀pLX007(SalI/XhoI), pCC009(SalI/XhoI) and lacZa(SalI/XhoI)
2倍体积乙醇, 1/10体积NaAc.
回收于20uL, 离心30min.
SDA-EGFP-pCC002(No.1,5)阳转长起来了
但提质粒用的是存下来的菌液, BamHI/XhoI(7uL/20uL)切检验, OK! 存质粒.
.gif)
还有菌液在冰箱门上存着, 用时直接提.
mRFP-PCC002, SDY-ECFP-pCC002的板子没有长, 昨天晚上连的体系转化
RD1-1-T, RD1-2-T转化
提取<bbpart>BBa_I13522</bbpart>(1-4)
等晚上提质粒. 试管上标记为GFP-1,2,3,4.
已提好.
pCC009(1,2,3,4), pLX007(4) XhoI单切检验(2uL/20uL)
lacZa(SalI/XhoI)电泳看浓度, 10uL, Marker 10uL (稍大)
连接
- lacZa-pLX007(4uL/1uL), H2O-pLX007自连
pCC009(1-4)XhoI.jpg)
- MCS-pCC009(4uL/1uL), H20-pCC009自连
File:PKU Switch 07-7-6 lazZa xs.gif
5pm-6pm: 4 degree, 6pm-9pm 16 degree.
