IGEM:Peking/2007/Switch-Notebook/2007-7-7: Difference between revisions
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共长20个, P出来5个 (没P出来的都是什么) | 共长20个, P出来5个 (没P出来的都是什么) | ||
===Colony PCR test of RD1-1,2-pMD18T=== | |||
昨天分区的没作阴性没有跑 | |||
Primer: MBF/R | |||
[[Image:PKU Switch 07-7-7 RD1-1 RD1-2.gif]] | |||
l to r: | |||
pMBD, RD1-1(1-12), RD1-2(1-11), ladder | |||
Only RD1-1(11) is OK. 连T的效率这么低, 好奇怪啊. | |||
再检一批. | |||
Revision as of 01:51, 7 July 2007
Experimenter: LXL, NM, CDZ
Contents
Sequencing result of SDE_EGFP_pMD18T received
Only No. 1 is correct.
Where is the plasmid? Culture?? Experiment notes???
等候处理.
Colony PCR test of ECFP-pCC002, mRFP-pCC002 (分区)
Primer
- ECFP: pKan_F, EGFP_EcoRV_R
- mRFP: pKan_F, mRFP_NotI_R
l to r: ECFP 1-12, 27-30, mRFP 13-20, Marker
l to r: mRFP 21-26, Marker
For Mini-prep tomorrow: ECFP 9, mRFP 14, 15, 21, 25, 26
mRFP看大小应该差不多, ECFP比较悬.
我也没有ClaI_SDY_F(Cla前没有保护碱基)的引物, 也不可能从T上往下切. 按理说CFP与mRFP也不差什么, 确认一下片段浓度, 没有问题就重连, 有问题就重切好了.
SD1,2-pMD18T grew very little
Primer: MBF/R
l to r: SD1(1-8), SD2(9-20), pMDB, pSul-pMD18T, RD1-2(12), ladder
For sequencing tomorrow: SD1(1,4), SD2(1,2,6)
共长20个, P出来5个 (没P出来的都是什么)
Colony PCR test of RD1-1,2-pMD18T
昨天分区的没作阴性没有跑
Primer: MBF/R
l to r: pMBD, RD1-1(1-12), RD1-2(1-11), ladder
Only RD1-1(11) is OK. 连T的效率这么低, 好奇怪啊.
再检一批.
