IGEM:Peking/2007/Switch-Notebook/2007-8-10

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Contents

rescue by precipitation

9 fragments: lac, rec, sul, SS1-3, SD1-3, digested by XhoI/SpeI

1 vector: GFP-plx007, digested by XhoI/XbaI

miniprep

T1TE-plx007

T1TE-T1T2-pcc010

double digestion test applied to these two plasmids

sequencing results

change the primers to ensure the right result!

transformation

Prec, Plac, Psul, SS1-3,SD1-3 ligated with GFP-plx007 for 5 hours

SDY-EGFP-plx007 positive transformation

re-colony PCR of T1TE-T1T2-pcc010

forget to make the positive control! failed.

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