IGEM:Peking/2007/Switch-Notebook/2007-8-11

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Contents

colony PCR of T1TE-T1T2-pcc010 and T1T2-pcc010 self-ligation

the results are strange and hard to explain, PCR always tricky???

re-PCR lac, rec, sul, ss1, ss2, ss3, sd1-3

rescue through gel and digest them

Image:PKU Switch 07-8-11 lac, rec etc.jpg

prepare some Amp+ LB plate

colony PCR of yesterday's plate

sula-GFP-plx007

Image:PKU Switch 07-8-11 sula.jpg

lac-GFP-plx007

Image:PKU Switch 07-8-11 lac.jpg

ss2-GFP-plx007

Image:PKU Switch 07-8-11 ss2.jpg

re-digestion of GFP-plx007

xbaI and XhoI

in the result, two bands through single enzyme digestion! Two sites for both XhoI and XbaI? seem to be impossible! maybe PCR or other procedure included some other DNA here, we need more thorough check about all these things!!!

re-PCR double promoters

positive transformation

to prepare for enough supplies

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