IGEM:Peking/2007/Switch-Notebook/2007-8-14: Difference between revisions
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Chen Daizhuo (talk | contribs) (New page: ==Ligation of SulA, Rec, SD1-3, SS1-2 with GFP-pLX007== ===Precipitate and retract=== SulA, Rec, SD1-3, SS1-2, GFP-pLX007 ===Ligate with GFP-pLX007=== SulA, Rec, SD1-3, SS1-2 ===Transfo...) |
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==Ligation of SulA, Rec, SD1-3, SS1-2 with GFP-pLX007== | ==Ligation of SulA, Rec, SD1-3, SS1-2 with GFP-pLX007== | ||
===Precipitate | ===Precipitate for retraction=== | ||
SulA, Rec, SD1-3, SS1-2, GFP-pLX007 | SulA, Rec, SD1-3, SS1-2, GFP-pLX007 (^^ with XbaI & XhoI) | ||
===Ligate with GFP-pLX007=== | ===Ligate with GFP-pLX007=== | ||
| Line 8: | Line 8: | ||
===Transformed in the evening=== | ===Transformed in the evening=== | ||
==Colony PCR test of Prec, SD2-3, SS1-3-(SDY-EGFP-pLX007)== | |||
Number of colonies grew up: | |||
<pre> | |||
Prec 0 | |||
SD2 0 | |||
SD3 1 | |||
SS1 >50 | |||
SS2 2 | |||
SS3 14 | |||
</pre> | |||
Only SS1 is right. 4 colonies are select for incubation. | |||
Revision as of 23:04, 17 August 2007
Ligation of SulA, Rec, SD1-3, SS1-2 with GFP-pLX007
Precipitate for retraction
SulA, Rec, SD1-3, SS1-2, GFP-pLX007 (^^ with XbaI & XhoI)
Ligate with GFP-pLX007
SulA, Rec, SD1-3, SS1-2
Transformed in the evening
Colony PCR test of Prec, SD2-3, SS1-3-(SDY-EGFP-pLX007)
Number of colonies grew up:
Prec 0 SD2 0 SD3 1 SS1 >50 SS2 2 SS3 14
Only SS1 is right. 4 colonies are select for incubation.
