IGEM:Peking/2007/Switch-Notebook/2007-8-14: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
Chen Daizhuo (talk | contribs) mNo edit summary |
Chen Daizhuo (talk | contribs) mNo edit summary |
||
| Line 24: | Line 24: | ||
Only SS1 is right. 4 colonies are select for incubation. | Only SS1 is right. 4 colonies are select for incubation. | ||
==Colony PCR test of T1T2-(Tet F-lacZ-pLX010)== | |||
6 colonies are selected for incubation. | |||
Revision as of 23:09, 17 August 2007
Ligation of SulA, Rec, SD1-3, SS1-2 with GFP-pLX007
Precipitate for retraction
SulA, Rec, SD1-3, SS1-2, GFP-pLX007 (^^ with XbaI & XhoI)
Ligate with GFP-pLX007
SulA, Rec, SD1-3, SS1-2
Transformed in the evening
Colony PCR test of Prec, SD2-3, SS1-3-(SDY-EGFP-pLX007)
Number of colonies grew up:
No. ---------- Prec 0 SD2 0 SD3 1 SS1 >50 SS2 2 SS3 14
Only SS1 is right. 4 colonies are select for incubation.
Colony PCR test of T1T2-(Tet F-lacZ-pLX010)
6 colonies are selected for incubation.
