IGEM:Peking/2007/Switch-Notebook/2007-8-14

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(New page: ==Ligation of SulA, Rec, SD1-3, SS1-2 with GFP-pLX007== ===Precipitate and retract=== SulA, Rec, SD1-3, SS1-2, GFP-pLX007 ===Ligate with GFP-pLX007=== SulA, Rec, SD1-3, SS1-2 ===Transfo...)
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==Ligation of SulA, Rec, SD1-3, SS1-2 with GFP-pLX007==
==Ligation of SulA, Rec, SD1-3, SS1-2 with GFP-pLX007==
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===Precipitate and retract===
+
===Precipitate for retraction===
-
SulA, Rec, SD1-3, SS1-2, GFP-pLX007
+
SulA, Rec, SD1-3, SS1-2, GFP-pLX007 (^^ with XbaI & XhoI)
===Ligate with GFP-pLX007===
===Ligate with GFP-pLX007===
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===Transformed in the evening===
===Transformed in the evening===
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 +
==Colony PCR test of Prec, SD2-3, SS1-3-(SDY-EGFP-pLX007)==
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 +
Number of colonies grew up:
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<pre>
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Prec  0
 +
SD2  0
 +
SD3  1
 +
SS1 >50
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SS2  2
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SS3  14
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</pre>
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 +
Only SS1 is right. 4 colonies are select for incubation.

Revision as of 02:04, 18 August 2007

Contents

Ligation of SulA, Rec, SD1-3, SS1-2 with GFP-pLX007

Precipitate for retraction

SulA, Rec, SD1-3, SS1-2, GFP-pLX007 (^^ with XbaI & XhoI)

Ligate with GFP-pLX007

SulA, Rec, SD1-3, SS1-2

Transformed in the evening

Colony PCR test of Prec, SD2-3, SS1-3-(SDY-EGFP-pLX007)

Number of colonies grew up:

Prec  0
SD2   0
SD3   1
SS1 >50
SS2   2
SS3  14

Only SS1 is right. 4 colonies are select for incubation.

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