IGEM:Peking/2007/Switch-Notebook/2007-8-14

Ligation of SulA, Rec, SD1-3, SS1-2 with GFP-pLX007

Precipitate for retraction

SulA, Rec, SD1-3, SS1-2, GFP-pLX007 (^^ with XbaI & XhoI)

Ligate with GFP-pLX007

SulA, Rec, SD1-3, SS1-2

Colony PCR test of Prec, SD2-3, SS1-3-(SDY-EGFP-pLX007)

Number of colonies grew up:

       No.
----------
Prec    0
SD2     0
SD3     1
SS1   >50
SS2     2
SS3    14

Only SS1 is right. 4 colonies are select for incubation.

Colony PCR test of T1T2-(Tet F-lacZ-pLX010)

6 colonies are selected for incubation.