IGEM:Peking/2007/Switch-Notebook/2007-8-15: Difference between revisions
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Chen Daizhuo (talk | contribs) (New page: ==Mini-prep and double digestion of T1TE-pLX010, SS1-(GFP-pLX007)== * T1TE-pLX010 ^^ with ScaI and XhoI. Right 552bp/Wrong 512bp * SS1-(GFP-pLX007) ^^ with SalI and XhoI. Right 896bp/Wrong...) |
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==Mini-prep and double digestion of T1TE-pLX010, SS1-(GFP-pLX007)== | ==Mini-prep and double digestion of T1TE-pLX010, SS1-(GFP-pLX007)== | ||
===T1TE-pLX010 ^^ with ScaI and XhoI=== | |||
[[Image:Peking-Switch-070815-T1TE-pLX010 ScaI XhoI.jpg]] | |||
l to r: | |||
T1TE-pLX010^^1-22, 100bp ladder, lambda marker | |||
Right 552bp/Wrong 512bp | |||
Select for sequencing: | |||
4, 8, 10, 11, 12, 14 | |||
===SS1-(GFP-pLX007) ^^ with SalI and XhoI=== | |||
[[Image:Peking-Switch-070815-SS1-GFPpLX007 SalI XhoI.jpg]] | |||
l to r: | |||
SS1-(GFP-pLX007)^^1-5, 100bp ladder, lambda marker | |||
Right 896bp/Wrong 756bp | |||
All are wrong.. | |||
Revision as of 00:21, 18 August 2007
Mini-prep and double digestion of T1TE-pLX010, SS1-(GFP-pLX007)
T1TE-pLX010 ^^ with ScaI and XhoI
l to r: T1TE-pLX010^^1-22, 100bp ladder, lambda marker
Right 552bp/Wrong 512bp
Select for sequencing: 4, 8, 10, 11, 12, 14
SS1-(GFP-pLX007) ^^ with SalI and XhoI
l to r: SS1-(GFP-pLX007)^^1-5, 100bp ladder, lambda marker
Right 896bp/Wrong 756bp
All are wrong..
