IGEM:Peking/2007/Switch-Notebook/2007-8-15

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(New page: ==Mini-prep and double digestion of T1TE-pLX010, SS1-(GFP-pLX007)== * T1TE-pLX010 ^^ with ScaI and XhoI. Right 552bp/Wrong 512bp * SS1-(GFP-pLX007) ^^ with SalI and XhoI. Right 896bp/Wrong...)
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==Mini-prep and double digestion of T1TE-pLX010, SS1-(GFP-pLX007)==
==Mini-prep and double digestion of T1TE-pLX010, SS1-(GFP-pLX007)==
-
* T1TE-pLX010 ^^ with ScaI and XhoI. Right 552bp/Wrong 512bp
+
===T1TE-pLX010 ^^ with ScaI and XhoI===
-
* SS1-(GFP-pLX007) ^^ with SalI and XhoI. Right 896bp/Wrong 756bp
+
 
 +
[[Image:Peking-Switch-070815-T1TE-pLX010 ScaI XhoI.jpg]]
 +
 
 +
l to r:
 +
T1TE-pLX010^^1-22, 100bp ladder, lambda marker
 +
 
 +
Right 552bp/Wrong 512bp
 +
 
 +
Select for sequencing:
 +
4, 8, 10, 11, 12, 14
 +
 
 +
===SS1-(GFP-pLX007) ^^ with SalI and XhoI===
 +
 
 +
[[Image:Peking-Switch-070815-SS1-GFPpLX007 SalI XhoI.jpg]]
 +
 
 +
l to r:
 +
SS1-(GFP-pLX007)^^1-5, 100bp ladder, lambda marker
 +
 
 +
Right 896bp/Wrong 756bp
 +
 
 +
All are wrong..

Revision as of 03:21, 18 August 2007

Mini-prep and double digestion of T1TE-pLX010, SS1-(GFP-pLX007)

T1TE-pLX010 ^^ with ScaI and XhoI

Image:Peking-Switch-070815-T1TE-pLX010 ScaI XhoI.jpg

l to r: T1TE-pLX010^^1-22, 100bp ladder, lambda marker

Right 552bp/Wrong 512bp

Select for sequencing: 4, 8, 10, 11, 12, 14

SS1-(GFP-pLX007) ^^ with SalI and XhoI

Image:Peking-Switch-070815-SS1-GFPpLX007 SalI XhoI.jpg

l to r: SS1-(GFP-pLX007)^^1-5, 100bp ladder, lambda marker

Right 896bp/Wrong 756bp

All are wrong..

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