IGEM:Peking/2007/Switch-Notebook/2007-8-15

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Contents

Mini-prep and double digestion of T1TE-pLX010, SS1-(GFP-pLX007)

T1TE-pLX010 ^^ with ScaI and XhoI

Image:Peking-Switch-070815-T1TE-pLX010 ScaI XhoI.jpg

l to r: T1TE-pLX010^^1-22, 100bp ladder, lambda marker

Right 552bp/Wrong 512bp

Select for sequencing: 4, 8, 10, 11, 12, 14

SS1-(GFP-pLX007) ^^ with SalI and XhoI

Image:Peking-Switch-070815-SS1-GFPpLX007 SalI XhoI.jpg

l to r: SS1-(GFP-pLX007)^^1-5, 100bp ladder, lambda marker

Right 896bp/Wrong 756bp

All are wrong..

Colony PCR test of SulA, Rec, lac, SS1-3, SD1-3-(GFP-pLX007)

Fragment      No. of colonies PCRed / selected
------------------------------------------------------------------------
SulA                             24 /  4
lac                              24 / 11
Rec                              22 /  2
SS1                              24 /  0
SS2                              13 /  0
SS3                              18 /  9
SD1                              24 /  2
SD2                              24 /  4
SD3                              13 /  0
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